Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5516618 | Steroids | 2017 | 6 Pages |
â¢First investigation of Cynanchum menarandrense.â¢Five new C-21 steroidal glycosides identified.â¢The pregnanes-containing fraction stimulates GLP-1 secretion in intestinal cells.â¢Results suggest a synergistic effect.
Five new pregnane-type steroidal glycosides, named menarandrosides A-E (1-2, 5-7) were isolated from the aerial parts of Cynanchum menarandrense, together with three known compounds, carumbelloside I (3), carumbelloside II (4), and pregnenolone-3-O-gentiobioside (8). Their structures were determined on the basis of spectroscopic analyses including NMR and mass spectrometry, reporting C-21 steroids glycosylated only by one or two glucose moieties. Compounds were then investigated for their potential to stimulate glucagon-like peptide-1 (GLP-1) secretion in intestinal cells; although none of the pure compounds had any influence, the fraction enriched in pregnanes exhibited a significant activity, suggesting a possible synergistic effect. Furthermore, none of the purified compounds affected cell viability.
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