Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5522160 | Journal of Microbiological Methods | 2017 | 6 Pages |
â¢We present a novel in vitro biofilm model, utilising a sodium alginate substratum.â¢It generates reproducible staphylococcal and non-staphylococcal biofilms.â¢It reliably quantifies biofilm eradication, as measured by reduction in cell counts.
In vitro biofilm assays are a vital first step in the assessment of therapeutic effectiveness. Current biofilm models have been found to be limited by throughput, reproducibility, and cost. We present a novel in vitro biofilm model, utilising a sodium alginate substratum for surface biofilm colony formation, which can be readily dissolved for accurate evaluation of viable organisms. The dissolving bead biofilm assay was evaluated using a range of clinically relevant strains. The reproducibility and responsiveness of the assay to an antimicrobial challenge was assessed using standardised methods. Cryo-scanning electron microscopy was used to image biofilm colonies. Biofilms were grown for 20Â h prior to testing. The model provides a reproducible and responsive assay to clinically-relevant antimicrobial challenges, as defined by established guidelines. Moreover cryo-scanning electron microscopy demonstrates that biofilm formation is localised exclusively to the alginate bead surface.Our results suggest that this simple model provides a robust and adaptable assay for the investigation of bacterial biofilms.