| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5522165 | Journal of Microbiological Methods | 2017 | 4 Pages |
Abstract
â¢Re-examination of the widely used live-dead staining methodâ¢Showing that cells with a high membrane potential take up propidium ions like dead cellsâ¢Explaining the uptake with the electrochemical properties of the propidium ion
Live-dead staining with propidium iodide can give erroneous results for bacteria showing high membrane potentials. We observed uptake of propidium ions across intact cell membranes for Dinoroseobacter shibae and Bacillus subtilis. Apparently, a high membrane potential facilitates breakthrough of the double-charged propidium ion and can mark viable cells as dead.
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Authors
Christian Kirchhoff, Heribert Cypionka,