Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5522181 | Journal of Microbiological Methods | 2017 | 5 Pages |
â¢A new LAMP assay targeting lipL32 gene of pathogenic Leptospira spp. was developed.â¢LipL32 LAMP we designed enables detection of 10 copies of L. interrogans.â¢LipL32 LAMP assay has a high diagnostic sensitivity (91.67%) and specificity (100%).
Leptospirosis, a global zoonotic disease, is often neglected but has a significant impact on human health. Here we reported a new loop mediated isothermal amplification (LAMP) assay targeting lipL32 gene of pathogenic Leptospira spp. Polymerase chain reaction (PCR), nested-PCR and real-time PCR assays were included in this study for the comparison of analytic and diagnostic sensitivity and specificity. LipL32 LAMP we designed enables detection of 10 copies of L. interrogans and has a higher diagnostic sensitivity (91.67%) and specificity (100%) than other PCR-based methods. The high sensitivity, specificity and flexible reaction conditions of the lipL32 LAMP assay makes it feasible for resource-limited countries and on-site application.