| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5522252 | Journal of Microbiological Methods | 2017 | 6 Pages |
â¢A novel protocol for infecting zebrafish larvae with S. Typhimurium by static immersion is described.â¢Our infection protocol in zebrafish allows live-cell imaging of Salmonella colonization and zebrafish immune responses.â¢Zebrafish larvae is a valid model for rapid, non-invasive real-time analysis of Salmonella infections in a vertebrate host.
The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune response induced by GFP-tagged Salmonella Typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. Our novel infection protocols in zebrafish allow live-cell imaging of Salmonella colonization.
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