| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5522280 | Journal of Microbiological Methods | 2017 | 8 Pages |
Abstract
â¢A novel method for inhibition of gene expression in Staphylococcus aureus using CRISPR/dCas9â¢The method is suitable for rapid knockdown gene expression.â¢The method is verified for repressing multiple genes simultaneously.
The understanding of the genetic mechanism of Staphylococcus aureus requires efficient tools, however, genetic manipulation in S. aureus is always laborious and time-consuming. Here we proposed a novel CRISPR/dCas9 interference method for the rapid knockdown of target genes. Furthermore, multiple genes can be repressed simultaneously by using this method.
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Authors
Xiaoyun Dong, Yingli Jin, Di Ming, Bangbang Li, Haisi Dong, Lin Wang, Tiedong Wang, Dacheng Wang,