Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5522294 | Journal of Microbiological Methods | 2017 | 4 Pages |
Abstract
We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Marie Vandermies, Olivia Denies, Jean-Marc Nicaud, Patrick Fickers,