Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5522338 | Journal of Microbiological Methods | 2017 | 6 Pages |
â¢We present a mild and efficient method for harvesting microbial cells on silica columnsâ¢The method allows non-contaminating harvest under oxic or anoxic conditions preserving microbial and enzymatic activityâ¢The method is adaptable to different culture volume and bacterial cell sizesâ¢Harvesting of extremely small microorganisms
Microbial cells vary widely in size, specific density, shearing resistance, oxygen sensitivity and abundance so that differential harvesting and washing procedures are needed to efficiently recover cells from dilute suspensions. We here describe a mild, simple, variable and cost-efficient method to harvest cells on columns packed with silica beads. The method collects and concentrates 40-98% of the cells preserving enzymatic activity and cell viability. The method can be applied for strictly anaerobic microorganisms, is scalable to different culture volumes and can be multiplexed in standardized systems. We see major application potential in harvesting small cells leaking through 0.2 μm filters, for harvesting strictly anaerobic cells and for differential harvesting of cells according to cell size using a gradient system.