Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5522344 | Journal of Microbiological Methods | 2017 | 5 Pages |
Abstract
â¢Real-time immuno-PCR (RT-I-PCR) was developed to detect mycobacterial PstS1.â¢A range of 10 ng/mL-1 pg/mL of PstS1 was detected by RT-I-PCR in TB patients.â¢This is the first report to quantify PstS1 by RT-I-PCR in TB samples.
A novel indirect real-time immuno-polymerase chain reaction (RT-I-PCR) assay, an evolution of I-PCR, was developed for the quantitative detection of Mycobacterium tuberculosis PstS1 (Rv0934) with a wide dynamic range of 10Â ng/mL to 1Â pg/mL in body fluids of tuberculosis (TB) patients, which may monitor the dynamics of disease.
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Authors
Suman Sharma, Ankush Raj, Netrapal Singh, Bhawna Dahiya, Abhishek Sheoran, Krishna B. Gupta, Promod K. Mehta,