| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5522395 | Journal of Microbiological Methods | 2017 | 4 Pages |
Abstract
A split-marker system for targeted gene deletion was developed for the model grass endophytic fungus Epichloë festucae. Compared to the conventional system that yields up to 25% homologous recombinants, the method resulted in 33-74% targeted deletions in E. festucae using as little as 1.5Â kb of targeting sequence.
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Authors
M Rahnama, N Forester, K G S U Ariyawansa, C R Voisey, L J Johnson, R D Johnson, D J Fleetwood,