| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5534533 | Molecular and Cellular Probes | 2017 | 8 Pages |
Abstract
Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan®-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on clinical samples, 178 nasopharyngeal aspirate specimens from pediatric cases were analyzed and HBoV genome was detected in 13 out of 178 patients with a viral load range between 1.6Â ÃÂ 103 and 9.4Â ÃÂ 107 copies/ml. These results indicated that this method could be used as an alternative technique for the diagnosis of HBoV infection.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cell Biology
Authors
Marco Ligozzi, Erica Diani, Federica Lissandrini, Rosanna Mainardi, Davide Gibellini,