Article ID Journal Published Year Pages File Type
5534564 Molecular and Cellular Probes 2017 4 Pages PDF
Abstract

•A rapid and sensitive AHPND-RPA assay was developed for the detection of the AHPND-causing agent of Vibrio owensii.•The AHPND-RPA assay detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C.•The AHPND-RPA assay showed 100% positive predictive value and negative predictive value for AHPND diagnosis.

A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis.

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