Short communicationA TaqMan-based qRT-PCR assay for Senecavirus A detection in tissue samples of neonatal piglets

•The study describes a fast, sensitive, and specific TaqMan-based qRT-PCR technique.•The assay was able to generate reproducible and reliable results.•This is an efficient technique for SVA diagnosis and assessment of SVA load in tissues.

This study describes a sensitive (1.3 × 101 genomic copies/μL) and specific TaqMan-based qRT-PCR assay able to detect and quantify SVA RNA in porcine biological samples. The technique represents an efficient tool for the virus diagnosis and assessment of SVA load in tissues of infected animals and for epidemiological studies.