Highly oxygenated lanostane-type triterpenoids and their bioactivity from the fruiting body of Ganoderma gibbosum

Eight new highly oxygenated lanostane triterpenes, gibbosic acids A–H (1–8), along with three known ones (9–11), were isolated from the fruiting body of Ganoderma gibbosum. The structures of new isolates were assigned by NMR and HRESIMS experiments. The absolute configurations of 1 were further confirmed by single crystal X-ray diffraction data and computational ECD methods. Immunoregulatory effect and anti-inflammatory activities of these compounds were screened in murine lymphocyte proliferation assay and in lipopolysaccharide (LPS)-stimulated RAW-264.7 macrophages, respectively. Compound 2 exhibited immunostimulatory effect both in lymphocyte proliferation assay without any induction and ConA-induced mitogenic activity of T-lymphocyte, and the proportion of lymphocyte proliferation at the concentration of 0.1 μM are 20.01% and 21.40%, respectively.

Graphical abstractEight new lanostane triterpenes, gibbosic acids A–H (1–8), were isolated from the fruiting body of Ganoderma gibbosum. Compound 2 exerted immunostimulatory effect both in Lymphocyte proliferation assay without any induction and ConA-induced mitogenic activity of T-lymphocyte.Figure optionsDownload full-size imageDownload high-quality image (189 K)Download as PowerPoint slide