Sarniensine, a mesembrine-type alkaloid isolated from Nerine sarniensis, an indigenous South African Amaryllidaceae, with larvicidal and adulticidal activities against Aedes aegypti

A new mesembrine-type alkaloid, named sarniensine, was isolated together with tazettine, lycorine, the main alkaloid, and 3-epimacronine from Nerine sarniensis, with the last two produced for the first time by this plant. This Amaryllidaceae, which is indigenous of South Africa, was investigated for its alkaloid content, because the organic extract of its bulbs showed strong larvicidal activity with an LC50 value of 0.008 μg μL− 1 against first instar Aedes aegypti larvae and with an LD50 value 4.6 μg/mosquito against adult female Ae. aegypti, which is the major vector for dengue, yellow fever and the Zika virus. The extract did not show repellency at MED value of 0.375 mg cm2 against adult Ae. aegypti. Sarniensine was characterized using spectroscopic and chiroptical methods as (3aR,4Z,6S,7aS)-6-methoxy-3a-(2′-methoxymethyl-benzo [1,3]dioxol-1'-yl)-1-methyl-2,3,3a,6,7,7a-hexahydro-1H-indole. It was less effective against larva at the lowest concentration of 0.1 μg μL− 1, however it showed strong adulticidal activity with an LD50 value of 1.38 ± 0.056 μg mosquito− 1.

Graphical abstractSarniensine, a new mesembrine-type alkaloid, was isolated together with three already known alkaloids from Nerine sarniensis bulbs, an Amaryllidaceae indigenous of South Africa. It showed strong adulticidal activity against Aedes aegypti the major vector of the arboviruses responsible for dengue, yellow fever, and Zika diseases.Figure optionsDownload full-size imageDownload high-quality image (165 K)Download as PowerPoint slide