Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5556562 | Journal of Pharmacological and Toxicological Methods | 2017 | 7 Pages |
IntroductionThe Göttingen minipig is a promising model for pharmacological safety assessment and for translational research in cardiology. We have examined the main ion currents in cardiomyocytes of the minipig heart.MethodsCardiac cells were isolated from different cardiac regions (endo-, mid- and epicardial left ventricle and right ventricle) from Göttingen minipigs and examined using the whole cell patch clamp technique combined with pharmacological interventions.ResultsThe inward rectifier (IK1), the delayed rectifier (IK), with the rapid and slow components, (IKr, IKs) and the L-type Ca2Â + channel (ICa,L) were identified in the different regions of the heart, whereas the Ca2Â +-independent transient outward current (Ito1) was observed in only a few cells. IK1 was similar in the cardiac regions with a slightly lower value in the epicardial cells. IKs was smaller in epi- and endo-cardial regions.DiscussionThe equivalents of the main human cardiac ion currents are present in the minipig cardiomyocytes with the exception of the Ca2Â +-independent Ito1. The study provides further evidence that the minipig is a valid model for investigating cardiovascular pharmacology.