Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5671233 | Anaerobe | 2017 | 4 Pages |
â¢Model strains to study the C. difficile binary toxin, CDT, were generated by deleting the PaLoc in R20291.â¢The gene encoding CdtR was deleted and re-integrated on the chromosome in the model strains.â¢In vitro Cytotoxicity assays were established to assess CDT-mediated virulence.â¢CdtR was required for the production of CDT to cytotoxic levels towards Vero cells.
Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ÎPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.