Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5671700 | Current Opinion in Microbiology | 2017 | 8 Pages |
â¢Gene transfer agents (GTAs) are phage-like particles mediating gene transfer.â¢The Rhodobacter capsulatus GTA (RcGTA) is produced by a subpopulation.â¢RcGTA-like particles are produced by several Alphaproteobacteria.â¢Natural transformation-like genes are required to receive donated DNA.â¢The response regulator CtrA controls both RcGTA production and recipient capability.
Gene transfer agents (GTAs) are bacteriophage-like particles produced by many prokaryotes. Several members of the Alphaproteobacteria produce a class of genetically-related GTAs that is best studied in Rhodobacter capsulatus. DNA transfer by the R. capsulatus GTA (RcGTA) combines aspects of both transduction and natural transformation, as recipient cells require a natural transformation-like system to incorporate donated DNA. The genes involved in RcGTA production and recipient capability are located at multiple loci in the bacterial genome; however, a conserved phosphorelay containing the response regulator CtrA and a quorum sensing system regulate both RcGTA production and recipient capability. This review highlights recent discoveries in RcGTA biology, and focuses on the co-regulation of genes involved in RcGTA production and recipient capability.
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