Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5671724 | Current Opinion in Microbiology | 2017 | 12 Pages |
â¢Two classes of CRISPR-Cas systems differ by the architectures of effector modules.â¢Effectors of Class 2 CRISPR-Cas systems are large, multidomain proteins.â¢Effector modules of Class 2 CRISPR-Cas systems independently evolved from transposon genes.â¢Some Class 2 CRISPR-Cas effectors are also involved in pre-crRNA provessing.â¢Dedicated RNA-targeting Class 2 CRISPR-cas systems employ HEPN domains for target cleavage.
The bacterial and archaeal CRISPR-Cas systems of adaptive immunity show remarkable diversity of protein composition, effector complex structure, genome locus architecture and mechanisms of adaptation, pre-CRISPR (cr)RNA processing and interference. The CRISPR-Cas systems belong to two classes, with multi-subunit effector complexes in Class 1 and single-protein effector modules in Class 2. Concerted genomic and experimental efforts on comprehensive characterization of Class 2 CRISPR-Cas systems led to the identification of two new types and several subtypes. The newly characterized type VI systems are the first among the CRISPR-Cas variants to exclusively target RNA. Unexpectedly, in some of the class 2 systems, the effector protein is additionally responsible for the pre-crRNA processing. Comparative analysis of the effector complexes indicates that Class 2 systems evolved from mobile genetic elements on multiple, independent occasions.