Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5673748 | Microbial Pathogenesis | 2017 | 16 Pages |
Abstract
Several risk factors have been linked to lung cancer (LC). Nevertheless, a viral etiology has been mentioned for a subset of patients developing LC. The aim of this study was to evaluate the effect of Merkel cell polyomavirus (MCPyV) on developing non-small cell lung cancer (NSCLCs). In total, 96 paraffin-embedded NSCLC biopsies and 96 adjacent non-LC normal specimens were analyzed by quantitative real-time polymerase chain reaction (PCR) for the existence of the MCPyV DNA and the expressions of RNA transcripts. Among the 96 enrolled participants, 42 patients were adenocarcinomas (ADs) and 54 patients were squamous cell carcinoma (SCC). Of the 42 ADs, MCPyV DNA was determined in 15 (35.7%) samples and of the 54 SCC, MCPyV DNA was detected in 22 (40.7%) samples. Only one non-cancerous sample in SCC subjects was positive for MCPyV LT-Ag DNA load (0.216Â ÃÂ 10â3). In MCPyV-positive subjects, the median MCPyV copy number was higher in the patients with ADs (0.016Â ÃÂ 10â3 copies/cell) compared to SCCs (0.005Â ÃÂ 10â3 copies/cell); but this difference was not statistically significant (PÂ =Â 0.913). In the seven stages of LC, the MCPyV LT-Ag was quantified in stage IV (0.204Â ÃÂ 10â3 copies/cell) more than in other stages. There was statistically significant difference between stages of cancer and MCPyV LT-Ag DNA load (PÂ =Â 0.002). These results revealed for the first time the presence of MCPyV in a subset of patients with NSCLCs in Iran. Further studies should be carried out to clarify the role of MCPyV in lung carcinogenesis.
Related Topics
Life Sciences
Immunology and Microbiology
Microbiology
Authors
Anahita Behdarvand, Mohammad Saber Zamani, Farzin Sadeghi, Yousef Yahyapour, Farzam Vaziri, Fatemeh Rahimi Jamnani, Bahareh Nowruzi, Abolfazl Fateh, Seyed Davar Siadat,