| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5673979 | Microbial Pathogenesis | 2017 | 5 Pages |
â¢About 50% of V. cholerae isolates from 2012 and 2013 outbreaks in Iran fulfilled the criteria of MDR phenotype.â¢The SXT element with its mosaic structure was the exclusive antimicrobial resistance determinant of cholera outbreaks of 2012 and 2013 in Iran.â¢Class 1 integron widely distributed among enteric bacteria, did not play an important role in resistance of Iranian V. cholerae strains.â¢All the isolates uniformly appeared to be identical in fingerprinting profiles expected from outbreak strains.
The objective of this study was to characterize antimicrobial resistance determinants in relation to antimicrobial susceptibility and genotyping profile in 20 clinical isolates of Vibrio cholerae. All of the isolates were resistant to streptomycin. The second most prevalent resistance was observed to trimethoprim (75%), co-trimoxazole (60%), tetracycline (50%), and minocycline (45%). About 50% of the isolates fulfilled the criteria of Multi Drug Resistance (MDR) phenotype. None of the isolates carried tet A, B, C, and, D determinants. This finding shows that tetracycline resistance determinants recognized so far, does not satisfactorily describe the 50% tetracycline resistance phenotype in this study, suggesting the possible contribution of other not yet characterized resistance mechanisms involved. Class 1 integron, widely distributed among enteric bacteria, was not detected among V. cholerae strains under study. Conversely, 100% of the isolates harbored SXT constin(int), among which 70% were positive for dfrA1, strA, and strB genes. The sul1gene was present in 60% of the isolates while none of them contained floR gene. All the isolates uniformly appeared to be identical in fingerprinting profiles expected from outbreak strains. In conclusion, SXT element with its mosaic structure was the exclusive antimicrobial resistance determinant of clonal V. cholerae isolates taken from outbreaks of 2012 and 2013 in Iran.
