Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5819704 | International Journal of Pharmaceutics | 2014 | 10 Pages |
Abstract
160Â nm nanocapsules containing up to 60% of camptothecin in the core and 7-8 polyelectrolyte bilayers in the shell were produced by washless layer-by-layer assembly of heparin and block-copolymer of poly-l-lysine and polyethylene glycol. The outer surface of the nanocapsules was additionally modified with polyethylene glycol of 5Â kDa or 20Â kDa molecular weight to attain protein resistant properties, colloidal stability in serum and prolonged release of the drug from the capsules. An advantage of the LbL coated capsules is the preservation of camptothecin lactone form with the shell assembly starting at acidic pH and improved chemical stability of encapsulated drug at neutral and basic pH, especially in the presence of albumin that makes such formulation more active than free camptothecin. LbL nanocapsules preserve the camptothecin lactone form at pH 7.4 resulting in triple activity of the drug toward CRL2303 glioblastoma cell.
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Authors
Gaurav Parekh, Pravin Pattekari, Chaitanya Joshi, Tatsiana Shutava, Mark DeCoster, Tatyana Levchenko, Vladimir Torchilin, Yuri Lvov,