A quality by design (QbD) case study on liposomes containing hydrophilic API: II. Screening of critical variables, and establishment of design space at laboratory scale

Contour plot for EE% with respect to lipid concentration and drug concentration. Red star points are central composite design points. Within the circle (red star points) is the design space for prediction of EE%. Additional data points (white disks) are included to evaluate the accuracy and robustness of the design space.