Novel non-peptide small molecules preventing IKKÎ²/NEMO association inhibit NF-ÎºB activation in LPS-stimulated J774 macrophages

Article ID	Journal	Published Year	Pages	File Type
5823198	Biochemical Pharmacology	2016	48 Pages	PDF

Keywords

NF-κB M-MLV RT Tris–borate–EDTA 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide SBVS J774 macrophages HTVS NBD IKK TBE NEMO iNOS ERK RT-qPCR GAPDH ADME PBS LPS DMEM NCI RMSD FBS DMSO IκB kinase MTT NO2−Electrophoretic mobility shift assay inflammation H-bonds absorption, distribution, metabolism, and excretion Dimethyl sulfoxide foetal bovine serum inducible nitric-oxide synthase EMSA یا electrophoretic mobility shift assay Virtual screening Structure-based virtual screening nuclear factor-κB lipopolysaccharide Phosphate-buffered saline Dulbecco’s modified eagle’s medium NF-κB essential modulator National Cancer Institute root mean square deviation Nitrite Nitric oxide Reverse Transcription-quantitative Polymerase Chain Reaction moloney murine leukemia virus reverse transcriptase Hydrogen bonds Molecular docking extracellular-signal-related kinase glyceraldehyde-3-phosphate dehydrogenase

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Health Sciences Pharmacology, Toxicology and Pharmaceutical Science Pharmacology

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Novel non-peptide small molecules preventing IKKÎ²/NEMO association inhibit NF-ÎºB activation in LPS-stimulated J774 macrophages

Authors

Francesca De Falco, Carmen Di Giovanni, Carmen Cerchia, Daniela De Stefano, Antonella Capuozzo, Carlo Irace, Teresa Iuvone, Rita Santamaria, Rosa Carnuccio, Antonio Lavecchia,