Ligustilide alleviates brain damage and improves cognitive function in rats of chronic cerebral hypoperfusion

NeuN expression in parietal cortex and in hippocampus in 2VO rats with or without LIG treatment by immunofluorescence. Panel A-C represents parietal cortex and panel D-F represents hippocampus. Panel A and D: Representative images of the SHAM group. Panel B and E: Representative images of the 2VO group. Panel C and F: Representative images of the LIG group. Panel II: Representative image of parietal cortex; and Panel III: Representative image of hippocampus. Black pane represents the detected areas of all brain slices. Panel G: A histogram representing the numbers of neuron in parietal cortex (0.27 mm2). Panel H: A histogram representing the percentage surface areas (shown as ratio of SHAM) of neurons in hippocampus CA1 (0.27 mm2). Data are presented as means±SEM. Significance levels are given as: 2VO vs. SHAM: ⁎*P<0.01; LIG vs. 2VO: ♯P<0.05. CA, the CA1 area of the hippocampus; LIG, Ligustilide; NeuN, Neuronal Nuclei; 2VO, Bilateral Common Carotid Artery Occlusion; PC, parietal cortex. 563