Effects of HuangKui capsules on glibenclamide pharmacokinetics in rats

EthnopharmacologyThe main components of HuangKui capsules' are the total flavonoids extracted from the flowers of Abelmoschus manihot L medic. They have been widely used to treat chronic glomerulonephritis, diabetic nephropathy and nephrotic syndrome. The combination of HuangKui capsules and glibenclamide is a possible therapy for patients with diabetes mellitus and diabetic nephropathy. However, there is no report about effects of HuangKui capsules on the glibenclamide pharmacokinetics till now.Aim of the studyThis study was aimed investigating the effect of HuangKui capsules on pharmacokinetics of glibenclamide in rats.Material and methodsEight rats were administered with an oral dose of HuangKui capsules (0.75 g kg−1) once daily for 10 consecutive days. All the rats were administered orally with the glibenclamide (1 mg kg−1) before the first time and after the last time given HuangKui capsules. LC-MS/MS was utilized to determine the concentration of glibenclamide in rat plasma and to calculate the corresponding pharmacokinetic parameters. The statistical differences of the two cycles were evaluated by paired-samples t-test.ResultsIn the rats treated with HuangKui capsules and glibenclamide, the t1/2, the time point of maximum plasma concentration (Tmax) of glibenclamide increased obviously (p < 0.05) compared with the glibenclamide alone, while maximum plasma concentrations (Cmax), area under the plasma concentration-time curve (AUC(0−t)) decreased significantly (p < 0.05). There was no significant difference between other parameters.ConclusionHuangKui capsules can reduce the absorption of glibenclamide and accelerate the metabolism of glibenclamide.

Graphical abstractIt showed that the plasma concentrations of glibenclamide in rats were significantly decreased when it was co-administrated with HuangKui capsules and Cmax, AUC(0−t) were decreased significantly, too.Download high-res image (179KB)Download full-size image