Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5839745 | Journal of Ethnopharmacology | 2011 | 10 Pages |
Abstract
Aim of the studyBaicalin is an active compound originating from the root of Scutellaria baicalensis Georgi, which has been used for anti-inflammation, anti-bacteria, anti-hypertension, anti-allergy and sedation since ancient China, though the neuronal mechanisms involved in the sedative effect is still unclear. Baicalin possesses the ability to decrease the expression of pro-inflammatory cytokines and nuclear factor (NF)-κB activity. Furthermore, baicalin has demonstrated an anxiolytic-like effect via activation of γ-aminobutyric acid-A (GABAA) receptors. Pro-inflammatory cytokines (e.g. interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF)-α) and the GABAergic system promote sleep. This study was designed to determine whether the GABAA receptor activation and/or the suppression of pro-inflammatory cytokines mediate(s) baicalin-induced sleep alterations.Materials and methodsBaicalin was intracerebroventricularly (ICV) administered 20 min either prior to the beginning of the light period or before the onset of the dark period. Electroencephalogram (EEG) and gross body movement were acquired for sleep analysis. Pharmacological blockade of IL-1 and GABAA receptors were employed to elucidate the involvements of IL-1 and GABAA receptors in baicalin-induced sleep alterations. IL-1β concentrations obtained after baicalin administration in several distinct brain regions were determined by ELISA.ResultsICV administration of baicalin decreased slow wave sleep (SWS) during the first 2 h of the light period. Rapid eye movement sleep (REMS) was not altered. The blockade of IL-1β-induced SWS enhancement by baicalin suggests that the antagonism of IL-1 receptors is involved in baicalin-induced SWS decrement during the light period. However, IL-1β concentrations during the light period were not altered after baicalin administration. In contrast, baicalin increased both SWS and REMS during hours 8-10 of the dark (active) period when baicalin was administered at the beginning of the dark period, and its effects were blocked by the GABAA receptor antagonist bicuculline.ConclusionBaicalin exhibits biphasic effects on sleep-wake regulation; the decrease of SWS during the light period and increases of SWS and REMS during the dark period. Inhibition of IL-1 action and enhancement of GABAA receptor activity may mediate baicalin's effects during the light and dark period, respectively.
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Authors
Han-Han Chang, Pei-Lu Yi, Chiung-Hsiang Cheng, Chin-Yu Lu, Yi-Tse Hsiao, Yi-Fong Tsai, Chia-Ling Li, Fang-Chia Chang,