| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5840399 | Journal of Pharmacological and Toxicological Methods | 2016 | 6 Pages |
Abstract
A sequence of elaborated protocol modifications allows subcellular localization analysis of Cav1.2 in an immunocytochemical approach. We provide a protocol that may be used to achieve insights into physiological and pathophysiological processes involving voltage gated calcium channels. Our protocol may be used for expression analysis of other challenging proteins and efficient overexpression may be exploited in related biochemical techniques requiring immunolabels.
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Authors
Peter Koch, Stefan Herzig, Jan Matthes,