Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5914234 | Journal of Structural Biology | 2014 | 7 Pages |
Abstract
Cryo-electron microscopy is an increasingly popular tool for studying the structure and dynamics of biological macromolecules at high resolution. A crucial step in automating single-particle reconstruction of a biological sample is the selection of particle images from a micrograph. We present a novel algorithm for selecting particle images in low-contrast conditions; it proves more effective than the human eye on close-to-focus micrographs, yielding improved or comparable resolution in reconstructions of two macromolecular complexes.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Molecular Biology
Authors
Robert Langlois, Jesper Pallesen, Jordan T. Ash, Danny Nam Ho, John L. Rubinstein, Joachim Frank,