| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 592820 | Colloids and Surfaces A: Physicochemical and Engineering Aspects | 2014 | 10 Pages |
•Synthesis and characterization of amine- and thiol-functionalized magnetic nanoparticles.•Chemisorption of metallocarbonyl complex to magnetic nanoparticles.•Biofunctionalization of metallocarbonyl magnetic nanoparticle conjugates with anti-rabbit IgG.•Characterization of the antibody-coated metallocarbonyl magnetic nanoparticles by IR spectroscopy and ELISA.
Metallocarbonyl complexes and their bioconjugates display specific and intense absorption bands in the mid-IR spectral range assigned to the stretching vibrations of the carbonyl ligands which make them useful bioprobes to study biomolecular processes. Here we report the design, synthesis, and characterization of amine- and thiol-functionalized magnetic nanoparticles (MNP) and their conjugates with the metallocarbonyl complex (η5-cyclopentadienyl)Fe(CO)2(η1-N-maleimidato). The resulting metallocarbonyl-labeled magnetic nanoparticles were derivatized with rabbit IgG to provide IR reporter immunoprobes. The antigen binding capability of the bioconjugates was confirmed by ELISA.
Graphical abstractThe metallocarbonyl-labeled magnetic nanoparticles were derivatized with rabbit IgG to provide IR reporter immunoprobes.Figure optionsDownload full-size imageDownload as PowerPoint slide
