Hydrogen nanobubbles in a water solution of dietary supplement

•We have measured the hydrogen gas yield from the dietary tablets dissolved in water.•We observed the oil emulsification due to the presence of nanobubbles.•We considered a simple model to estimate the lifetime of nanobubbles.•NMR proton spectra were measured at different times after adding the tablet to the water.•Proton NMR is a useful method to estimate the volume and lifetime of nanobubbles.

Using gas chromatography, proton nuclear magnetic resonance and qualitative experiments, we demonstrate that a water solution of dissolved dietary supplement, creating negative redox potential, contains invisible hydrogen nano-bubbles, which remain in the solution for several hours.

Graphical abstractHydrogen, as revealed by basic and clinical research, is an important physiological regulatory factor producing antioxidant, anti-inflammatory and anti-apoptotic protective effects on cells and organs. We provide experimental evidence that a dietary supplement, designed to saturate water with hydrogen, produces long-lived hydrogen nanobubbles. Using gas chromatography, proton nuclear magnetic resonance and qualitative experiments, we demonstrate that a water solution of dissolved dietary supplement, creating negative redox potential, contains invisible hydrogen nano-bubbles, which remain in the solution for several hours. Figure optionsDownload full-size imageDownload as PowerPoint slide