| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 594730 | Colloids and Surfaces A: Physicochemical and Engineering Aspects | 2011 | 7 Pages |
In this study the potential applications of manganese ferrite (MnFe2O4) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe2O4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Manganese ferrite nanoparticles conjugated to an anti Sticholysin monoclonal antibody. ► Peroxidase-like activity of manganese ferrite nanoparticles. ► Bioanalytical applications of manganese ferrite nanoparticles. ► Immunoassay and protocol for protein separation using manganese ferrite nanoparticles.
