Probing site-selective binding of rhodamine B to bovine serum albumin

▶ With the objective of probing binding mechanisms of rhodamine dye with protein, the interaction of rhodamine B and bovine serum albumin was investigated by the complementary spectroscopic methods. ▶ Results showed that the binding of rhodamine B to bovine serum albumin was concentrations-dependently site-selective binding. ▶ Rhodamine molecules preferentially bound to site I of protein when dye concentrations were low, whereas dye molecules selectively bound to site II of protein at their higher concentration.