Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
595473 | Colloids and Surfaces A: Physicochemical and Engineering Aspects | 2009 | 6 Pages |
Abstract
In this study the effects of houttuyfonate homologues (HOU-Cn) on the surface and shape, the lipid fluidity and the protein conformation, the fatty acid compositions and fatty acid synthase II (FAS II) of B. subtilis were studied to elucidate the antibacterial mechanism of HOU-Cn against Bacillus subtilis. The scanning electronic microscope (SEM) results showed that the glycocalyx on the surface of B. subtilis disappeared and the cell became smaller after being treated with HOU-Cn. During the co-incubation of HOU-Cn and B. subtilis, HOU-Cn was decomposed into alkyl acyl aldehyde and sodium sulfite rapidly. Then alkyl acyl aldehyde was congregated onto cell membrane and inserted into lipid bilayer, further increased the fluidity of membrane and changed the conformation of membrane protein by hydrophobic binding. Subsequently, HOU-Cn inhibited the FAS II activity, and decreased the synthesis of fatty acids, especially increased the percentage of saturated fatty acid. HOU-Cn showed a strong antibacterial activity against G+-bacteria by a multi-target antibacterial mechanism.
Keywords
Bacillus subtilisDPHPHENADPHL-phenylalaninePBS1,6-diphenyl-1,3,5-hexatrieneEDTAethylene diamine tetraacetic acidFatty acid compositionMembrane fluidityfatty acid methyl estersFAME یا fatty acid methyl esters phosphate buffer solutionSEMScanning electronic microscopenicotinamide adenine dinucleotide phosphate
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Xiaoli Ye, Li Xu, Xuegang Li, Zhu Chen, Baoshun Zhang, Lujiang Yuan, Xing Chen, Huajin Zhang, Wen Chang, Shengliang Sun,