| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 596110 | Colloids and Surfaces A: Physicochemical and Engineering Aspects | 2009 | 7 Pages |
Abstract
The interaction between bovine hemoglobin (BHb) and Fe3O4 colloid was investigated by UV/vis absorption, FT-IR, fluorescence, resonance light scattering spectra, CD, synchronous fluorescence, and three-dimensional fluorescence spectra techniques under physiological pH 7.40. Fe3O4 effectively quenched the intrinsic fluorescence of BHb via static quenching. The process of binding Fe3O4 on BHb was a spontaneous molecular interaction procedure. The thermodynamic parameters indicate that van der Waals and hydrogen bonds, electrostatic interactions played a major role in stabilizing the Fe3O4–BHb complex. The effect of Fe3O4 on the conformation of BHb was analyzed using CD and synchronous fluorescence spectroscopy.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Yan-Qing Wang, Hong-Mei Zhang, Qiu-Hua Zhou, Hai-Lan Xu,