Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6020107 | Journal of Neuroimmunology | 2015 | 9 Pages |
Abstract
Our previous work has shown that cerebellar interposed nucleus (IN) modulates immune function. Herein, we reveal mechanism underlying the immunomodulation. Treatment of bilateral cerebellar IN of rats with 3-mercaptopropionic acid (3-MP), a glutamic acid decarboxylase antagonist that reduces γ-aminobutyric acid (GABA) synthesis, enhanced cellular and humoral immune responses to bovine serum albumin, whereas injection of vigabatrin, a GABA-transaminase inhibitor that inhibits GABA degradation, in bilateral cerebellar IN attenuated the immune responses. The 3-MP or vigabatrin administrations in the cerebellar IN decreased or increased hypothalamic GABA content and lymphoid tissues' norepinephrine content, respectively, but did not alter adrenocortical or thyroid hormone levels in serum. In addition, a direct GABAergic projection from cerebellar IN to hypothalamus was found. These findings suggest that GABAergic neurons in cerebellar IN regulate immune system via hypothalamic and sympathetic pathways.
Keywords
3, 3′, 5, 5′-tetramethylbenzidinePBSACTHInterposed nucleusPBMCsConcanavalin ALHAPHATMBTSHSCP3-MPBSAbovine serum albuminsuperior cerebellar peduncle3-Mercaptopropionic acidγ-aminobutyric acidkainic acidCellular immunityHumoral immunityinterferonIFNinterleukinCon Aphosphate bufferELISAEnzyme-linked immunosorbent assayDHAperipheral blood mononuclear cellsFluoro-RubynorepinephrineDentate nucleusFastigial nucleusadrenocorticotropic hormonethyroid stimulating hormoneoptical densityhigh performance liquid chromatographyHPLCGABA
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Authors
Jian-Hua Lu, Xiao-Qin Wang, Yan Huang, Yi-Hua Qiu, Yu-Ping Peng,