Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6020403 | Journal of Neuroimmunology | 2014 | 13 Pages |
Abstract
Subcutaneous or intraperitoneal administration of Toll-like receptor (TLR)-9 agonist, ODN 1668 caused moderate fever and anorexia. In comparison to stimulation of other intracellular TLRs, activation of TLR9 did not result in pronounced peripheral induction of interferons, but rather induced interleukin-6. Expression of cytokines (TNFα, IL-1β) and inducible forms of enzymes for prostaglandin E2 synthesis occurred in the brain, in conjunction with a moderate activation of the transcription factors STAT3 and NF-IL6 in brain endothelial cells. The lack of a septic-like state in ODN 1668-treated rats reinforces the therapeutic value of this drug.
Keywords
vWFCVOsTLRPAMPODNCpGOVLTIκBαPGE2SOCS3RT-PCRNF-IL6COX-2Microsomal prostaglandin E synthaseOligodeoxynucleotidesmPGESTNFPBSinhibitor kappa B alphaSTAT3NFκBorganum vasculosum laminae terminalisEnzyme-Linked Immuno-Sorbent Assaycircumventricular organsImmunohistochemistryIHCinterferonIFNinterferonsinterleukinFeverELISAToll-like receptorstandard errorsuppressor of cytokine signaling 3CytokinesCyclooxygenase-2Immune-to-brain signalingarea postremaVon Willebrand factortumor necrosis factornuclear factor kappa BPhosphate buffered salinesignal transducer and activator of transcription 3Real time polymerase chain reactionpathogen associated molecular patternProstaglandin E2Toll-like receptors
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Authors
J. Damm, F. Wiegand, L.M. Harden, S. Wenisch, R. Gerstberger, C. Rummel, J. Roth,