Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6020621 | Journal of Neuroimmunology | 2013 | 7 Pages |
Abstract
Transferrin (Tf) has a major role in T cell activation and proliferation. Here, we investigated whether Tf exerts immunomodulatory effects on T cells and in development of T-cell driven experimental autoimmune encephalomyelitis (EAE). While treatment of concanavalin A-stimulated splenocytes with apotransferrin (ApoTf) did not affect release of IL-1β, TNF, IFN-γ, IL-17, IL-4, and IL-10, it markedly and dose-dependently down-regulated synthesis of IL-2 in these cells. ApoTf also inhibited IL-2 generation in purified CD3+ T cells and the effect was accompanied with down-regulation of MAPK p44/42 and NFκB signaling. Despite impeded IL-2 release, proliferation of splenocytes was not inhibited by ApoTf. Importantly, ApoTf ameliorated EAE in mice and significantly reduced ex vivo IL-2 production in proteolipid protein-specific lymphocytes. Thus ApoTf may be a promising beneficial agent for multiple sclerosis.
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Authors
Tamara Saksida, Djordje Miljkovic, Gordana Timotijevic, Ivana Stojanovic, Sanja Mijatovic, Paolo Fagone, Katia Mangano, Santa Mammana, Claudio Farina, Ester Ascione, Valentina Maiello, Ferdinando Nicoletti, Stanislava Stosic-Grujicic,