| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6021207 | Journal of Neuroimmunology | 2011 | 5 Pages |
Abstract
Antibodies to MOG in serum have a dubious prognostic value in multiple sclerosis. The MOG recombinant protein conformational properties relevant to the antigenic activity are unknown. We employed a solid-phase ELISA based on a product (rMOGED(His)6) expressed in E. coli after subcloning the cDNA of the extracellular domain of rat MOG, performing a refolding procedure on column and affinity purification. The far-UV Circular Dichroism (CD) spectra of rMOGED(His)6 showed a β-sheet, a characteristic feature of the Ig-fold. However, in MS sera and controls we failed to detected IgM or IgG antibodies.
Related Topics
Life Sciences
Immunology and Microbiology
Immunology
Authors
Francesca Gori, Barbara Mulinacci, Lara Massai, Carlo Avolio, Mariantonietta Caragnano, Elisa Peroni, Silvia Lori, Mario Chelli, Anna Maria Papini, Paolo Rovero, Francesco Lolli,