Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6021226 | Journal of Neuroimmunology | 2011 | 4 Pages |
Abstract
We investigated the overlap shared between the immunoglobulin (Ig) proteome of the cerebrospinal fluid (CSF) and the B cell Ig-transcriptome of CSF and the central nervous system (CNS) tissue of three patients with multiple sclerosis. We determined the IgG-proteomes of CSF by mass spectrometry, and compared them to the IgG-transcriptomes from CSF and brain lesions, which were analyzed by cDNA cloning. Characteristic peptides that were identified in the CSF-proteome could also be detected in the transcriptomes of both, brain lesions and CSF, providing evidence for a strong overlap of the IgG repertoires in brain lesions and in the CSF.
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Authors
Birgit Obermeier, Laura Lovato, Reinhard Mentele, Wolfgang Brück, Ignasi Forne, Axel Imhof, Friedrich Lottspeich, Katherine W. Turk, Simon N. Willis, Hartmut Wekerle, Reinhard Hohlfeld, David A. Hafler, Kevin C. O'Connor, Klaus Dornmair,