| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 606951 | Journal of Colloid and Interface Science | 2014 | 9 Pages |
•The doxorubicin loaded nanocarriers were fabricated for assaying U251 cell apoptosis.•The drug-loaded nanocarriers could efficiently inhibit proliferation of U251 cells.•The drug-loaded nanocarriers could effectively induce apoptosis of U251 cells.•The constructed nanocarriers were promising vehicles for drug delivery to U251 cells.
Magnetic nanoparticles (MNPs), in comparison with traditional drug solutions or suspensions, represent a promising vehicle to achieve the controlled drug delivery to targeted cell/tissue regions in cancer treatment. In this study, the biodegradable chitosan-modified magnetite (Fe3O4) NPs (CS-MNPs) are firstly synthesized using as nanocarriers, and then encapsulated with anti-tumor drug doxorubicin (DOX) to construct DOX-loaded CS-MNPs (DOX-CS-MNPs), which are further applied to assay apoptosis of glioblastoma multiforme U251 cells. The properties of the DOX-CS-MNPs including particle size, shape and magnetization, are characterized. The stability, drug release, magnetic response and redispersion of the DOX-CS-MNPs within an external magnetic field are evaluated. Furthermore, the biological effects of the DOX-CS-MNPs on U251 glioblastoma cells, particularly cytotoxicity, cell viability, actin cytoskeleton and apoptosis rate, are subsequently investigated. The data show that the prepared DOX-CS-MNPs are spherical in shape with average diameter of 60 nm approximately. The fabricated DOX-CS-MNPs also exhibit specific properties including low aggregation, high saturation magnetization, satisfactory magnetic-responsive aggregation, and redispersion in water, etc. The biological assays show that the DOX-CS-MNPs can efficiently enter the cells, reduce cell viability, and inhibit cell proliferation in a dose-dependent manner, and a high rate of cell apoptosis is induced in U251 glioblastoma cells after DOX-CS-MNPs treatment. Therefore, the present results indicate that the constructed DOX-CS-MNPs may be a promising vehicle for efficiently inhibiting proliferation of human U251 glioblastoma cells.
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