Preparation of polydiacetylene immobilized optically encoded beads

Polydiacetylene (PDA), which can change the chromic and fluorescence properties by inducing environmental perturbations, is immobilized on planar solid supports for many biological applications. In this work, we immobilize PDA onto optically encoded spherical beads (PDA–SERS beads). The prepared PDA immobilized beads (36 μm) exhibit a blue color without fluorescence. By inducing stress, their color and fluorescence properties are changed to red with fluorescence. The SERS spectra of the PDA–SERS beads can be recognized over the PDA background. Moreover, our PDA immobilization methods are successfully applied to silica-surface SERS-encoded beads (5 μm) and proven to also be useful in fluorescence encoding systems.

Graphical abstractPDA immobilized encoded beads (PDA–SERS beads, PDA–FL beads) which have their encoding capacity and PDA properties, which cause their optical properties to be changed, are prepared.Figure optionsDownload full-size imageDownload high-quality image (60 K)Download as PowerPoint slideResearch highlights► A novel PDA immobilization method for optically encoded micro beads which have their encoding capacity and PDA properties was developed. ► Their optical properties of PDA immobilized encoded beads, which can be changed their property by external stress, was studied. ► The immobilization methods was successfully applied to SERS-encoded beads and proven to also be useful in fluorescence encoding systems.