Lipid membranes supported on optically transparent nanosilicas: Synthesis and application in characterization of protein-membrane interactions

We describe methods of preparation of lipid membranes supported on optically transparent nanosilicas (20 and 30 nm diameter) and an initial application of these materials to characterize membrane-protein binding using conventional circular dichroism and fluorescence spectroscopy.

Graphical abstractLipid bilayers supported on ∅20 nm (left) and ∅100 nm (right) silicas. The amount of adsorbed lipids is ∼7 mg/sample in each vial.Figure optionsDownload full-size imageDownload high-quality image (54 K)Download as PowerPoint slideResearch highlights► We describe preparation of lipid membranes supported on optically transparent nanosilicas (∅ 20 and 30 nm). ► These materials combine the properties of solid-supported membranes (easy handling and improved stability) and those of lipid vesicles (high surface area and optical transparency) providing a convenient system for characterization of membrane-protein interactions using simple techniques. ► The interactions of melittin with lipid-coated nanosilicas are investigated using conventional CD and fluorescence spectroscopy.