Fluorescence of commercial Pluronic F127 samples: Temperature-dependent micellization

We present a novel approach of using the butylated hydroxytoluene (BHT) antioxidant found in commercial Pluronic F127 samples as a marker of polymer aggregation. The BHT marker was compared to the pyrene dye and static light scattering methods as a way to measure the critical micelle concentration (CMC) and critical micelle temperature (CMT). The n → π∗ transitions of BHT are sensitive to the microenvironment as demonstrated by plotting the fractional intensities of its excitation (≈280 nm) and emission (≈325 nm) peaks. BHT is more sensitive to changes in temperature than concentration. The partition coefficient increases ≈40-fold for pyrene compared to ≈2-fold for BHT when the temperature is increased from 25 to 37 °C. CMT values determined using the BHT fluorescence decrease with increasing F127 concentration. Our results show that BHT can be used as a reliable marker of changes in the microenvironment of Pluronic F127.

Graphical abstractBHT antioxidant in Pluronic F127 can be used to probe polymer aggregation.Figure optionsDownload full-size imageDownload high-quality image (77 K)Download as PowerPoint slideResearch highlights► Antioxidant BHT fluorescence sensitive to Pluronic F127 microenvironment. ► BHT fluorescence increases as it partitions into Pluronic F127 micellar phase. ► BHT fluorescence used to measure critical micelle temperature.