Fabrication of mesoporous SiO2–C–Fe3O4/γ–Fe2O3 and SiO2–C–Fe magnetic composites

A synthetic method for the fabrication of silica-based mesoporous magnetic (Fe or iron oxide spinel) nanocomposites with enhanced adsorption and magnetic capabilities is presented. The successful in situ synthesis of magnetic nanoparticles is a consequence of the incorporation of a small amount of carbon into the pores of the silica, this step being essential for the generation of relatively large iron oxide magnetic nanocrystals (∼10 ± 3 nm) and for the formation of iron nanoparticles. These composites combine good magnetic properties (superparamagnetic behaviour in the case of SiO2–C–Fe3O4/γ–Fe2O3 samples) with a large and accessible porosity made up of wide mesopores (>9 nm). In the present work, we have demonstrated the usefulness of this kind of composite for the adsorption of a globular protein (hemoglobin). The results obtained show that a significant amount of hemoglobin can be immobilized within the pores of these materials (up to 180 mg g−1 for some of the samples). Moreover, we have proved that the composite loaded with hemoglobin can be easily manipulated by means of an external magnetic field.

Graphical abstractThis work shows the fabrication of (silica)–(carbon)–(iron oxide or iron) magnetic composites and their application to the manipulation of biomolecules.Figure optionsDownload full-size imageDownload as PowerPoint slide