The interaction between casein micelles and gold nanoparticles

For the first time, the interaction of casein micelle (CM) with gold nanoparticles (GNPs) was studied using TEM, UV–visible, FTIR and fluorescence spectroscopy methods. The results show that GNPs bind to CM surface, leading to the formation of GNP–CM conjugates. GNPs exhibit similar particle diameters, and the CM structure is retained in GNP–CM conjugates. Furthermore, GNP–CM conjugates display good stability against salt concentration and pH. The combination of these measurements suggests that GNPs presumably bind to CM surfaces via complexation with the carboxylate or amine groups on CM surfaces, but not by electrostatic action.

Graphical abstractDiagram illustrating gold nanoparticles binding on casein micelles.Figure optionsDownload full-size imageDownload as PowerPoint slide