| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 612022 | Journal of Colloid and Interface Science | 2007 | 7 Pages |
Abstract
Bio-functionalization of silica nanoparticles was performed by covalent binding of penicillin G acylase. Structure-function analysis of free and bound enzyme using CD and activity tests revealed only minimal changes in secondary structure but an up to 80% decrease in specific activity upon immobilization. This may be caused rather by diffusion limitation than by a conformational change of the enzyme.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Bertolt Kranz, Jochen Bürck, Matthias Franzreb, Rainer Köster, Anne S. Ulrich,