Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6120411 | Journal of Clinical Virology | 2015 | 7 Pages |
Abstract
The percentage of dengue IgM/IgG ELISA positive cases that were negative by the nested RT-PCR varied from 17% to 42% for all serotypes depending on the year. Using TaqMan rRT-PCR, dengue RNA was detected in 194 (61%) of the 318 acute sera or extracted RNA previously found to be negative by the nested RT-PCR. The newly designed DENV-1 specific primer increased the sensitivity of DENV-1 detection by the nested RT-PCR from 48% to 88%, and of all 4 serotypes from 73% to 87%. These findings demonstrate the impact of genetic diversity and signal erosion on the sensitivity of PCR-based methods.
Keywords
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Authors
Chonticha Klungthong, Wudtichai Manasatienkij, Thipwipha Phonpakobsin, Piyawan Chinnawirotpisan, Prinyada Rodpradit, Kittinun Hussem, Butsaya Thaisomboonsuk, Prapapun Ong-ajchaowlerd, Ananda Nisalak, Siripen Kalayanarooj, Darunee Buddhari,