| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 612071 | Journal of Colloid and Interface Science | 2007 | 5 Pages |
Abstract
We report an alternative method for electrophoretic separation of large DNAs using steric confinement between solid walls, without gel or obstacles. The change of electrophoretic mobility vs confinement thickness is investigated using fluorescence video microscopy. We observe separation at small confinement thicknesses followed by a transition to the bulk behavior (no separation) at a thickness of about 4 μm (a few radii of gyration for the studied DNA chains). We present tentative explanations of our original observations.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Jérôme Mathé, Jean-Marc Di Meglio, Bernard Tinland,