Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6120985 | Journal of Clinical Virology | 2013 | 5 Pages |
BackgroundPCR coupled to electrospray ionization mass spectrometry technology (PCR/ESI-TOF-MS) (PLEX-ID system, Abbott Ibis Biosciences) was developed to characterize microbial pathogens.ObjectivesTo evaluate the performance of the PLEX-ID flu detection⢠kit for detecting Influenza viruses by comparison with the multiplex RespiFinder® Kit (PathoFinder).Study designAcute-phase respiratory samples (n = 293) were analysed for this purpose. A subpopulation of influenza type A positive samples, identified with the RespiFinder® kit (n = 64), were subtyped with the RealTime ready Inf A/H1N1 Detection Set® (Roche Molecular Diagnostics) and results were compared to the PLEX-ID Flu Detection⢠kit.Results274 samples gave concordant results (93.5%, p < 0.0001): 65 influenza A-positive, 18 influenza B-positive and 191 negative samples. Of these, 7 samples were PLEX-ID positive/RespiFinder® negative (5 influenza A and 2 influenza B) and 12 were PLEX-ID positive/RespiFinder® negative (10 influenza A and 2 influenza B). PLEX-ID showed one sample as an influenza A and B co-infection while the RespiFinder® assay showed it to be influenza A-positive. The sensitivity, specificity, positive and negative predictive values of the PLEX-ID⢠system were 87.4%, 96.5%, 92.2% and 94.1% respectively. Thirteen of 19 discordant samples available for retesting were investigated further with the Anyplexâ¢II RV16 Detection kit (Seegene): seven were RespiFinder® concordant, while six were PLEX-ID⢠concordant. Subtyping of 61/64 influenza A samples was concordant (95.3%): 55 were H1N1pdm09 and six were non-H1N1pdm09. Three samples gave negative PLEX-ID⢠results (one H1N1pdm09 and two non-H1N1pdm09).ConclusionsPCR/ESI-TOF-MS technology showed good diagnostic performances to detect and subtype influenza viruses.